Iikhowa ezinkulu zineseti etyebileyo neyahlukeneyo yee-metabolites ezisebenzayo kwaye zithathwa njengemithombo ebalulekileyo ye-bioresources. I-Phellinus igniarius yikhowa enkulu esetyenziswa ngokwesiko kwiinjongo zonyango kunye nokutya, kodwa udidi lwayo kunye negama lesiLatini zisaqhubeka ziphikisana. Besebenzisa uhlalutyo lokulungelelaniswa kwe-multigene segment, abaphandi baqinisekisile ukuba i-Phellinus igniarius kunye neentlobo ezifanayo ziyinxalenye yohlobo olutsha kwaye baseka uhlobo lwe-Sanghuangporus. Ikhowa le-honeysuckle i-Sanghuangporus lonicericola yenye yeentlobo ze-Sanghuangporus ezichongiweyo kwihlabathi liphela. I-Phellinus igniarius itsale ingqalelo enkulu ngenxa yeempawu zayo ezahlukeneyo zonyango, kubandakanya ii-polysaccharides, ii-polyphenols, ii-terpenes, kunye nee-flavonoids. Ii-Triterpenes zezona zinto zibalulekileyo ezisebenzayo kwi-pharmacologically kolu hlobo, zibonisa imisebenzi ye-antioxidant, antibacterial, kunye ne-antitumor.
IiTriterpenoids zinamandla amakhulu okusetyenziswa kwezorhwebo. Ngenxa yokunqaba kwezixhobo zeSanghuangporus zasendle kwindalo, ukuphucula ngempumelelo ukusebenza kwayo kunye nemveliso kubaluleke kakhulu. Okwangoku, kwenziwe inkqubela phambili ekuphuculeni imveliso yeemetabolites ezahlukeneyo zesibini zeSanghuangporus ngokusebenzisa ii-inducers zeekhemikhali ukulawula amaqhinga okubila angaphantsi komhlaba. Umzekelo, ii-polyunsaturated fatty acids, ii-fungal elicitors11 kunye ne-phytohormones (kubandakanya i-methyl jasmonate kunye ne-salicylic acid14) zibonakalisiwe ukuba zonyusa imveliso ye-triterpenoid eSanghuangporus. Abalawuli bokukhula kwezityalo(ii-PGR)inokulawula i-biosynthesis yee-metabolites zesibini kwizityalo. Kolu phononongo, i-PBZ, umlawuli wokukhula kwezityalo osetyenziswa kakhulu ukulawula ukukhula kwezityalo, isivuno, umgangatho kunye neempawu zomzimba, iphandwe. Ngokukodwa, ukusetyenziswa kwe-PBZ kunokuchaphazela indlela ye-terpenoid biosynthetic kwizityalo. Ukudibana kwe-gibberellins kunye ne-PBZ kwandisa umxholo we-quinone methide triterpene (QT) kwiMontevidia floribunda. Ukwakheka kwendlela ye-terpenoid yeoyile ye-lavender kutshintshwe emva konyango nge-400 ppm PBZ. Nangona kunjalo, akukho ngxelo malunga nokusetyenziswa kwe-PBZ kwiikhowa.
Ukongeza kwizifundo ezigxile ekwandeni kwemveliso ye-triterpene, ezinye izifundo zikwacacise iindlela zokulawula i-triterpene biosynthesis kwi-Moriformis phantsi kwempembelelo yeekhemikhali ezibangela iintsholongwane. Okwangoku, izifundo zigxile ekutshintsheni kwamanqanaba okubonakaliswa kwezakhi zofuzo ezinxulumene ne-triterpene biosynthesis kwindlela ye-MVA, ekhokelela ekwandeni kwemveliso ye-terpenoid.12,14 Nangona kunjalo, iindlela eziphantsi kwezi zakhi zofuzo zaziwayo, ingakumbi izinto ezibhaliweyo ezilawula ukubonakaliswa kwazo, azikacaci kwiindlela zokulawula i-triterpene biosynthesis kwi-Moriformis.
Kolu phononongo, iziphumo zoxinzelelo olwahlukeneyo lwe-plant growth regulators (PGRs) kwimveliso ye-triterpene kunye nokukhula kwe-mycelial ngexesha lokubila kwe-honeysuckle (S. lonicericola) engaphantsi komhlaba ziphandwe. Emva koko, i-metabolomics kunye ne-transcriptomics zisetyenzisiwe ukuhlalutya ukwakheka kwe-triterpene kunye neepatheni zokubonakaliswa kwe-gene ezibandakanyeka kwi-triterpene biosynthesis ngexesha lonyango lwe-PBZ. Idatha ye-RNA-sequencing kunye ne-bioinformatics ichonge ngakumbi i-target transcription factor ye-MYB (SlMYB). Ngaphezu koko, kwenziwe ii-mutants ukuqinisekisa isiphumo sokulawula se-SlMYB gene kwi-triterpene biosynthesis kunye nokuchonga ii-target genes ezinokubakho. I-Electrophoretic mobility shift assays (EMSA) zisetyenzisiwe ukuqinisekisa ukusebenzisana kwe-SlMYB protein kunye nabakhuthazi be-SlMYB target genes. Ngamafutshane, injongo yolu phononongo yayikukukhuthaza i-triterpene biosynthesis kusetyenziswa i-PBZ kunye nokuchonga i-MYB transcription factor (SlMYB) elawula ngokuthe ngqo ii-genes ze-triterpene biosynthetic kuquka i-MVD, i-IDI, kunye ne-FDPS kwi-S. lonicericola ukuphendula kwi-PBZ induction.
Ukungeniswa kwe-IAA kunye ne-PBZ kunyuse kakhulu imveliso ye-triterpenoid kwi-honeysuckle, kodwa isiphumo sokungeniswa kwe-PBZ sasibonakala ngakumbi. Ke ngoko, i-PBZ ifunyenwe iyeyona inducer ilungileyo kwi-concentration eyongezelelweyo ye-100 mg/L, nto leyo efanele ukuphandwa ngakumbi.
Ixesha lokuthumela: Agasti-19-2025