Amakhowa amakhulu aneseti etyebileyo neyohlukeneyo yeemetabolites ze-bioactive kwaye athathwa njengemithombo exabisekileyo yezinto eziphilayo. I-Phellinus igniarius likhowa elikhulu ngokwesiko elisetyenziselwa iinjongo zonyango kunye nokutya, kodwa ukuhlelwa kwayo kunye negama lesiLatini lihlala liphikisana. Ukusebenzisa uhlalutyo lwecandelo le-multigene, abaphandi baqinisekisile ukuba i-Phellinus igniarius kunye neentlobo ezifanayo ziyinxalenye entsha kwaye yaseka uhlobo lwe-Sanghuangporus. I-honeysuckle mushroom i-Sanghuangporus lonicericola yenye yeentlobo ze-Sanghuangporus ezichongiweyo kwihlabathi jikelele. I-Phellinus igniarius itsale umdla omkhulu ngenxa yeempawu zayo ezahlukeneyo zonyango, kubandakanya i-polysaccharides, iipolyphenols, i-terpenes, kunye ne-flavonoids. I-Triterpenes ziikhompawundi eziphambili ze-pharmacologically ezisebenzayo zolu hlobo, zibonisa imisebenzi ye-antioxidant, i-antibacterial, kunye ne-antitumor.
I-Triterpenoids inamandla amakhulu kwisicelo sorhwebo. Ngenxa yokunqaba kwemithombo yasendle ye-Sanghuangporus kwindalo, ukomeleza ngempumelelo i-biosynthetic yayo kunye nesivuno kubaluleke kakhulu. Okwangoku, inkqubela yenziwe ekuphuculeni imveliso yee-metabolites ezahlukeneyo zesibini ze-Sanghuangporus ngokusebenzisa ii-inducers zekhemikhali ukulawula amaqhinga okuvubela ngaphantsi kwamanzi. Ngokomzekelo, i-polyunsaturated fatty acids, i-fungi elicitors11 kunye ne-phytohormones (kubandakanya i-methyl jasmonate kunye ne-salicylic acid14) iboniswe ukwandisa imveliso ye-triterpenoid e-Sanghuangporus. Izilawuli zokukhula kwezityalo(PGRs)inokulawula i-biosynthesis ye-metabolites yesibini kwizityalo. Kule sifundo, i-PBZ, umlawuli wokukhula kwezityalo osetyenziswa ngokubanzi ukulawula ukukhula kwezityalo, isivuno, umgangatho kunye neempawu ze-physiological, zaphandwa. Ngokukodwa, ukusetyenziswa kwePBZ kunokuchaphazela indlela ye-terpenoid biosynthetic kwizityalo. Ukudityaniswa kwe-gibberellins kunye ne-PBZ kwandisa umxholo we-quinone methide triterpene (QT) eMontevidia floribunda.Ukwakheka kwendlela ye-terpenoid yeoli ye-lavender yatshintshwa emva konyango nge-400 ppm PBZ. Nangona kunjalo, akukho ngxelo malunga nokusetyenziswa kwePBZ kumakhowa.
Ukongeza kwizifundo ezijolise ekonyukeni kwemveliso ye-triterpene, ezinye izifundo ziye zacacisa iindlela zokulawula i-triterpene biosynthesis eMoriformis phantsi kweempembelelo zeekhemikhali. Okwangoku, izifundo zijolise ekuguquleni amanqanaba okubonakaliswa kwezakhi zofuzo ezihambelana ne-triterpene biosynthesis kwindlela ye-MVA, ekhokelela ekunyuseni kwemveliso ye-terpenoid.12,14 Nangona kunjalo, iindlela eziphantsi kwezi zakhi zofuzo ezaziwayo, ngokukodwa izinto ezibhaliweyo ezilawula ukubonakaliswa kwazo, zihlala zingacacanga kwiindlela zokulawula i-tripenesis biosynthesis.
Kolu phononongo, iimpembelelo zogxininiso olwahlukeneyo lwabalawuli bokukhula kwezityalo (PGRs) kwimveliso ye-triterpene kunye nokukhula kwe-mycelial ngexesha lokuvutshelwa kwamanzi e-honeysuckle (S. lonicericola) zaphandwa. Emva koko, i-metabolomics kunye ne-transcriptomics zisetyenziselwa ukuhlalutya ukubunjwa kwe-triterpene kunye neepatheni zokubonisa imfuza ezibandakanyekayo kwi-triterpene biosynthesis ngexesha lonyango lwe-PBZ. Ulandelelwano lwe-RNA kunye nedatha ye-bioinformatics ichonge ngakumbi into ekujoliswe kuyo yokukhutshelwa kwe-MYB (SlMYB). Ngaphaya koko, ii-mutants zenziwa ukuze ziqinisekise isiphumo solawulo se-SlMYB gene kwi-triterpene biosynthesis kunye nokuchonga imfuza ekujoliswe kuyo. Ii-Electrophoretic Mobility shift assays (EMSA) zisetyenziselwe ukuqinisekisa ukusebenzisana kweprotein ye-SlMYB kunye nabakhuthazi be-SlMYB ejoliswe kwiijini. Isishwankathelo, injongo yolu phononongo yayikukuvuselela i-triterpene biosynthesis usebenzisa i-PBZ kunye nokuchonga i-MYB transcription factor (SlMYB) elawula ngokuthe ngqo i-triterpene biosynthetic genes kuquka i-MVD, i-IDI, kunye ne-FDPS kwi-S. lonicericola ekuphenduleni i-PBZ induction.
Ukungeniswa kwe-IAA kunye ne-PBZ kwandisa kakhulu imveliso ye-triterpenoid kwi-honeysuckle, kodwa umphumo we-PBZ wawubonakala ngakumbi. Ngoko ke, i-PBZ yafunyanwa iyona nto i-inducer ingcono kwi-concentration eyongezelelweyo ye-100 mg / L, efanelwe ukufundwa okuqhubekayo.
Ixesha lokuposa: Aug-19-2025