Abaphandi abavela kwiSebe leBiochemistry kwiZiko leSayensi laseIndiya (IISc) bafumene indlela ekudala ifunwaukulawula ukukhula kwezityalo zomhlaba zakudala ezifana ne-bryophytes (iqela elibandakanya ubulembu kunye ne-liverworts) eyagcinwa kwizityalo ezidubulayo kamva.
Uphononongo, olupapashwe kwijenali ye-Nature Chemical Biology, igxininise ekulawuleni okungeyona i-canonical yeeprotheni ze-DELLA, umlawuli wokukhula oyintloko ocinezela ukuhlukana kweeseli kwi-embryophytes (izityalo zomhlaba).
Okubangela umdla kukuba, iibryophytes, izityalo zokuqala ezavela emhlabeni malunga ne-500 yezigidi zeminyaka eyadlulayo, azinayo i-GID1 receptor, nangona zivelisa i-phytohormone GA. Oku kuphakamisa umbuzo wokuba kwakulawulwa njani ukukhula nophuhliso lwezi zityalo zamandulo.
Ukusebenzisa i-liverwort Marchantia polymorpha njengenkqubo yemodeli, abaphandi bafumanisa ukuba ezi zityalo zamandulo zisebenzisa i-enzyme ekhethekileyo, i-MpVIH, eyenza i-cell messenger inositol pyrophosphate (InsP₈), ukudiliza i-DELLA ngaphandle kokubandakanyekagibberellik acid.
Abaphandi basebenzise inkqubo ye-CRISPR-Cas9 ukunkqonkqoza i-gene encoding i-enzyme ye-VIH, eqinisekisa indima yayo. Izityalo ezingenayo i-VIH esebenzayo zibonise iziphene ezinzulu zophuhliso kunye nokungahambi kakuhle kwe-morphological, njengamagqabi adibeneyo, ukukhula kwe-radial okungahambi kakuhle, kunye nokungabikho kwe-calyxes. Ezi ziphene zapheliswa ngokuguqula i-genome yesityalo ukuvelisa kuphela isiphelo esisodwa (i-N-terminus) ye-enzyme ye-VIH. Ukusebenzisa iindlela eziphambili ze-chromatography, iqela lifumene ukuba i-N-terminus iqulethe i-kinase domain eyenza ukuveliswa kwe-InsP₈.
Abaphandi bafumanisa ukuba i-DELLA yenye yeethagethi zeselula ze-VIH kinase. Ngaphezu koko, baqaphela ukuba i-phenotypes yezityalo ezinqongopheleyo ze-MpVIH zazifana nezo zezityalo ze-M. polymorpha kunye nokunyuka kwe-DELLA ibonakaliso.
"Okwangoku, sasinovuyo lokuqonda ukuba ukuzinza kwe-DELLA okanye umsebenzi uyaphuculwa kwizityalo ezinqongopheleyo ze-MpVIH," utshilo uPriyanshi Rana, umbhali wokuqala kunye nomfundi ophumeleleyo kwiqela lophando likaLahey. Ngokuhambelana ne-hypothesis yabo, abaphandi bafumanisa ukuba i-DELLA inhibition inokubuyisela ngokubonakalayo ukukhula okungahambi kakuhle kunye nophuhliso lwe-phenotypes yezityalo eziguqukayo ze-MpVIH. Ezi ziphumo zibonisa ukuba i-VIH kinase ilawula kakubi i-DELLA, ikhuthaza ukukhula kwezityalo kunye nophuhliso.
Uphando kwiiproteni ze-DELLA luqale kuHlaziyo loHlaza, xa izazinzulu ziye zasebenzisa ngokungaziyo amandla azo okudala iintlobo ezivelisa isivuno esiphezulu se-semi-dwarf. Nangona iinkcukacha zomsebenzi wabo zazingacacanga ngelo xesha, ubuchwephesha bale mihla buvumela izazinzulu ukuba zilawule imisebenzi yezi proteni ngobunjineli bemfuza, ngokufanelekileyo ukwandisa izivuno zezityalo.
Ixesha lokuposa: Oct-23-2025