Abaphandi abavela kwiSebe leBiochemistry kwi-Indian Institute of Sciences (IISc) bafumanise indlela ekudala ifunwayokulawula ukukhula kwezityalo zamandulo ezifana ne-bryophytes (iqela eliquka ii-mosses kunye ne-liverworts) ezagcinwa kwizityalo ezidubula kamva.
Olu phononongo, olupapashwe kwiphephancwadi iNature Chemical Biology, lugxile kulawulo olungelulo olusemthethweni lweeproteni zeDELLA, umlawuli oyintloko wokukhula othintela ukwahlukana kweeseli kwi-embryophytes (izityalo zomhlaba).
Okubangel’ umdla kukuba, ii-bryophytes, izityalo zokuqala ukuvela emhlabeni malunga neminyaka eyi-500 yezigidi eyadlulayo, azinayo i-GID1 receptor, nangona ivelisa i-phytohormone GA. Oku kuphakamisa umbuzo wokuba ukukhula nophuhliso lwezi zityalo zokuqala zomhlaba lwalulawulwa njani.
Besebenzisa i-liverwort Marchantia polymorpha njengenkqubo yomzekelo, abaphandi bafumanise ukuba ezi zityalo zamandulo zisebenzisa i-enzyme ekhethekileyo, i-MpVIH, evelisa i-cellular messenger inositol pyrophosphate (InsP₈), ukuze ichithe i-DELLA ngaphandle kokubandakanyeka kwei-gibberellic acid.
Abaphandi basebenzise inkqubo yeCRISPR-Cas9 ukucima i-gene ebhala i-enzyme ye-VIH, beqinisekisa indima yayo. Izityalo ezingenayo i-VIH esebenzayo zibonakalise iziphene ezinkulu zophuhliso kunye nokungahambi kakuhle kwesimo, njengamagqabi amancinci, ukukhula okuphazamisekileyo kwemitha, kunye nokungabikho kwee-calyxes. Ezi ziphene zisuswe ngokuguqula i-genome yesityalo ukuze kuveliswe isiphelo esinye kuphela (i-N-terminus) ye-enzyme ye-VIH. Besebenzisa iindlela eziphambili ze-chromatography, iqela lifumanise ukuba i-N-terminus ine-kinase domain ekhuthaza ukuveliswa kwe-InsP₈.
Abaphandi bafumanise ukuba i-DELLA yenye yeethagethi zeeseli ze-VIH kinase. Ngaphezu koko, baqaphele ukuba ii-phenotypes zezityalo ezingenayo i-MpVIH zifana nezo zezityalo ze-M. polymorpha kunye nokwanda kokubonakaliswa kwe-DELLA.
“Ngeli xesha, besinomdla wokuqonda ukuba ingaba uzinzo okanye umsebenzi weDELLA uyaphuculwa na kwizityalo ezingenayo iMpVIH,” utshilo uPriyanshi Rana, umbhali wokuqala kunye nomfundi ophumeleleyo kwiqela lophando likaLahey. Ngokuhambelana nengcamango yabo, abaphandi bafumanise ukuba ukuthintelwa kweDELLA kunokubuyisela kakhulu ii-phenotypes zokukhula nophuhliso oluneziphene kwizityalo eziguqukileyo zeMpVIH. Ezi ziphumo zibonisa ukuba iVIH kinase iyilawula kakubi iDELLA, ikhuthaza ukukhula nophuhliso lwezityalo.
Uphando malunga neeproteni zeDELLA luqala kwiNguquko yeGreen, xa izazinzulu bezisebenzisa amandla azo ngokungazi ukwenza iintlobo ze-semi-dwarf ezivelisa kakhulu. Nangona iinkcukacha zomsebenzi wazo zazingacacanga ngelo xesha, ubuchwepheshe banamhlanje buvumela izazinzulu ukuba zilawule imisebenzi yezi proteni ngokusebenzisa ubunjineli bemfuza, nto leyo eyandisa ngempumelelo isivuno sezityalo.
Ixesha leposi: Okthobha-23-2025