I-Uniconazoleyi-triazoleumlawuli wokukhula kwezityaloesetyenziswa kakhulu ukulawula ubude besityalo nokuthintela ukukhula kakhulu kwezithole. Nangona kunjalo, indlela yemolekyuli i-uniconazole ethintela ngayo ukunwebeka kwe-hypocotyl yezithole ayikacaci, kwaye kukho izifundo ezimbalwa kuphela ezidibanisa idatha ye-transcriptome kunye ne-metabolome ukuphanda indlela yokunwebeka kwe-hypocotyl. Apha, sibonile ukuba i-uniconazole ithintele kakhulu ukunwebeka kwe-hypocotyl kwizithole zekhaphetshu ezityatyambileyo zaseTshayina. Okunomdla kukuba, ngokusekelwe kuhlalutyo oludibeneyo lwe-transcriptome kunye ne-metabolome, sifumanise ukuba i-uniconazole ichaphazele kakhulu indlela "ye-phenylpropanoid biosynthesis". Kule ndlela, i-gene enye kuphela yosapho lwe-enzyme regulatory gene, i-BrPAL4, ebandakanyeka kwi-lignin biosynthesis, yancitshiswa kakhulu. Ukongeza, uvavanyo lwe-yeast one-hybrid kunye ne-two-hybrid lubonise ukuba i-BrbZIP39 inokubopha ngokuthe ngqo kummandla we-promoter we-BrPAL4 kwaye isebenze ukuguqulelwa kwayo. Inkqubo yokuthulisa iijini ezibangelwa yintsholongwane ibonakalise ngakumbi ukuba i-BrbZIP39 inokulawula ngokufanelekileyo ukunwebeka kwekhaphetshu yaseTshayina kunye nokwenziwa kwe-hypocotyl lignin. Iziphumo zolu phononongo zibonelela ngolwazi olutsha malunga nendlela yokulawula iimolekyuli ze-cloconazole ekuthinteleni ukunwebeka kwekhaphetshu yaseTshayina. Kwaqinisekiswa okokuqala ukuba i-cloconazole inciphise umxholo we-lignin ngokuthintela ukwenziwa kwe-phenylpropanoid okubangelwa yi-BrbZIP39-BrPAL4 module, ngaloo ndlela ikhokelele kwi-hypocotyl dwarfing kwizithole zekhaphetshu yaseTshayina.
Ikhaphetshu yaseTshayina (iBrassica campestris L. ssp. chinensis var. utilis Tsen et Lee) yeyohlobo lweBrassica kwaye sisityalo esidumileyo sonyaka esikhula kakhulu kwilizwe lam (Wang et al., 2022; Yue et al., 2022). Kwiminyaka yakutshanje, ubungakanani bemveliso yekholifulawa yaseTshayina buqhubekile nokukhula, kwaye indlela yokulima itshintshile ukusuka ekuhlwayeleni imbewu ngokuthe ngqo kwendabuko ukuya ekukhuliseni izithole ezinzulu kunye nokufakelwa kwazo. Nangona kunjalo, kwinkqubo yokukhulisa izithole ezinzulu kunye nokufakelwa kwazo, ukukhula okugqithisileyo kwe-hypocotyl kudla ngokuvelisa izithole ezinde, nto leyo ebangela umgangatho ophantsi wezithole. Ke ngoko, ukulawula ukukhula okugqithisileyo kwe-hypocotyl yingxaki ebalulekileyo ekukhuliseni izithole ezinzulu kunye nokufakelwa kwazo ikhaphetshu yaseTshayina. Okwangoku, zimbalwa izifundo ezidibanisa idatha ye-transcriptomics kunye ne-metabolomics ukuze kuhlolwe indlela yokwandiswa kwe-hypocotyl. Indlela ye-molecular apho i-chlorantazole ilawula ngayo ukwanda kwe-hypocotyl kwikhaphetshu yaseTshayina ayikafundwa. Sijonge ukuchonga ukuba zeziphi iijini kunye neendlela zeemolekyuli ezisabela kwi-hypocotyl dwarfing ebangelwa yi-uniconazole kwikhaphetshu yaseTshayina. Sisebenzisa uhlalutyo lwe-transcriptome kunye ne-metabolomic, kunye nohlalutyo lwe-yeast one-hybrid, uvavanyo lwe-dual luciferase, kunye novavanyo lwe-virus-induced gene silencing (VIGS), sifumanise ukuba i-uniconazole inokubangela i-hypocotyl dwarfing kwikhaphetshu yaseTshayina ngokuthintela i-lignin biosynthesis kwizithole zekhaphetshu yaseTshayina. Iziphumo zethu zibonelela ngolwazi olutsha malunga nendlela yokulawula iimolekyuli apho i-uniconazole ithintela khona i-hypocotyl elongation kwikhaphetshu yaseTshayina ngokuthintela i-phenylpropanoid biosynthesis elawulwa yi-BrbZIP39–BrPAL4 module. Ezi ziphumo zinokuba nemiphumo ebalulekileyo yokuphucula umgangatho wezithole zorhwebo kunye negalelo ekuqinisekiseni isivuno kunye nomgangatho wemifuno.
I-BrbZIP39 ORF epheleleyo yafakwa kwi-pGreenll 62-SK ukuvelisa i-effector, kwaye iqhekeza le-promoter le-BrPAL4 ladityaniswa kwi-pGreenll 0800 luciferase (LUC) reporter gene ukuvelisa i-reporter gene. Ii-effector kunye nee-reporter gene vectors zaguqulwa zaba ngamagqabi ecuba (i-Nicotiana benthamiana).
Ukuze sicacise ubudlelwane phakathi kwee-metabolites kunye nee-genes, senze uhlalutyo oludibeneyo lwe-metabolome kunye ne-transcriptome. Uhlalutyo lokuphucula indlela ye-KEGG lubonise ukuba ii-DEG kunye nee-DAM zityebiswe kunye kwiindlela ezingama-33 ze-KEGG (Umfanekiso 5A). Phakathi kwazo, indlela ye-"phenylpropanoid biosynthesis" yayiyeyona ityebiswe kakhulu; indlela ye-"photosynthetic carbon fixation", indlela ye-"flavonoid biosynthesis", indlela ye-"pentose-glucuronic acid interconversion", indlela ye-"tryptophan metabolism", kunye nendlela ye-"starch-sucrose metabolism" nazo zityebiswe kakhulu. Imephu yokuqokelela ubushushu (Umfanekiso 5B) ibonise ukuba ii-DAM ezinxulumene nee-DEG zahlulwe zibe ziindidi ezahlukeneyo, phakathi kwazo ii-flavonoids zaziziindidi ezinkulu, nto leyo ebonisa ukuba indlela ye-"phenylpropanoid biosynthesis" idlale indima ebalulekileyo kwi-hypocotyl dwarfism.
Ababhali bathi uphando lwenziwe ngaphandle kobudlelwane bezorhwebo okanye bezemali obunokuthathwa njengongquzulwano olunokwenzeka lwemidla.
Zonke izimvo ezivezwe kweli nqaku zezombhali kuphela kwaye azibonisi ngokuyimfuneko izimvo zemibutho enxulumeneyo, abapapashi, abahleli, okanye abahlaziyi. Naziphi na iimveliso ezivavanyiweyo kweli nqaku okanye amabango enziwe ngabavelisi bazo aziqinisekiswanga okanye azivunywa ngumshicileli.
Ixesha leposi: Matshi-24-2025