Uniconazoleyi triazoleumlawuli wokukhula kwezityaloesetyenziswa kakhulu ukulawula ubude besityalo nokuthintela ukukhula kwezithole. Nangona kunjalo, indlela yeemolekyuli apho i-uniconazole inqanda i-seedling hypocotyl elongation ayikacaci, kwaye kukho izifundo ezimbalwa ezidibanisa idatha ye-transcriptome kunye ne-metabolome ukuphanda indlela yokwandisa i-hypocotyl. Apha, siye saqaphela ukuba i-uniconazole inqanda kakhulu i-hypocotyl elongation kwizithole zekhaphetshu ezidubulayo zaseTshayina. Kuyathakazelisa ukuba, ngokusekelwe kwi-transcriptome edibeneyo kunye nohlalutyo lwe-metabolome, sifumene ukuba i-uniconazole ichaphazele kakhulu indlela "ye-phenylpropanoid biosynthesis". Kule ndlela, enye kuphela i-gene ye-enzyme yokulawula i-gene yentsapho, i-BRPAL4, ebandakanyekayo kwi-lignin biosynthesis, yathotywa kakhulu. Ukongeza, igwele lomxube omnye kunye novavanyo lwemihlanganiselwa emibini lubonise ukuba i-BrbZIP39 inokubophelela ngokuthe ngqo kwingingqi yomgqugquzeli we-BRPAL4 kwaye ivule ushicilelo lwayo. Inkqubo yokuthulisa imfuza ebangelwa yintsholongwane iphinde yangqina ukuba i-BrbZIP39 inokulawula ngokufanelekileyo ukolulwa kwekhaphetshu yaseTshayina kunye ne-hypocotyl lignin synthesis. Iziphumo zolu phononongo zibonelela ngemibono emitsha kwindlela yokulawula imolekyuli ye-cloconazole ekunqandeni ukwanda kwe-hypocotyl yekhaphetshu yaseTshayina. Kuye kwaqinisekiswa okokuqala ukuba i-cloconazole inciphisa umxholo we-lignin ngokunqanda ukuhlanganiswa kwe-phenylpropanoid elamlwa yimodyuli ye-BrbZIP39-BrPAL4, nto leyo ekhokelela kwi-hypocotyl dwarfing kwizithole zekhaphetshu zaseTshayina.
Ikhaphetshu yaseTshayina (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) yeyohlobo lweBrassica kwaye yimifuno eyaziwayo yonyaka yecruciferous ekhuliswa kakhulu kwilizwe lam (Wang et al., 2022; Yue et al., 2022). Kwiminyaka yakutshanje, isikali semveliso yecauliflower yaseTshayina iqhubekile nokukhula, kwaye indlela yokulima itshintshile ukusuka kwimbewu yemveli ethe ngqo ukuya kwinkcubeko yezithole kunye nokutshintshwa. Nangona kunjalo, kwinkqubo yenkcubeko ye-seedling enzulu kunye nokutshintshwa, ukukhula okugqithisileyo kwe-hypocotyl kudla ukuvelisa izithole ezimilenze, okukhokelela kumgangatho ophantsi wezithole. Ke ngoko, ukulawula ukukhula okugqithisileyo kwe-hypocotyl ngumba ocinezelayo kwinkcubeko yezithole kunye nokutshintshwa kwekhaphetshu yaseTshayina. Okwangoku, kukho izifundo ezimbalwa ezidibanisa i-transcriptomics kunye nedatha ye-metabolomics ukuphonononga indlela yokwandisa i-hypocotyl. Indlela yemolekyuli apho i-chlorantazole ilawula ukwandiswa kwe-hypocotyl kwikhaphetshu yaseTshayina ayikafundwa. Sijonge ukuchonga ukuba zeziphi iijini kunye neendlela zemolekyuli eziphendula kwi-hypocotyl dwarfing ye-uniconazole kwikhaphetshu yaseTshayina. Ukusebenzisa uhlalutyo lwe-transcriptome kunye ne-metabolomic, kunye nohlalutyo lwe-yeast one-hybrid, i-dual luciferase assay, kunye ne-virus-induced gene silenced (VIGS) assay, sifumene ukuba i-uniconazole inokubangela ukuba i-hypocotyl ibe mncinci kwikhaphetshu yaseTshayina ngokunqanda i-lignin biosynthesis kwimbewu yekhaphetshu yaseTshayina. Iziphumo zethu zibonelela ngemibono emitsha kwindlela yokulawula imolekyuli apho i-uniconazole inqanda ukwanda kwe-hypocotyl kwikhaphetshu yaseTshayina ngokuthintela i-phenylpropanoid biosynthesis elamlwa yimodyuli ye-BrbZIP39-BrPAL4. Ezi ziphumo zinokuba neempembelelo ezibalulekileyo ekuphuculeni umgangatho wezithole zorhwebo kunye negalelo ekuqinisekiseni isivuno kunye nomgangatho wemifuno.
Ubude obugcweleyo be-BrbZIP39 ORF yafakwa kwi-pGreenll 62-SK ukuvelisa i-effector, kunye neqhekeza lomxhasi we-BRPAL4 lidityaniswe kwi-pGreenll 0800 luciferase (LUC) ye-reporter gene ukwenza i-reporter gene. I-effector kunye ne-reporter gene vectors ziye zatshintshwa zibe yicuba (Nicotiana benthamiana) amagqabi.
Ukucacisa ubudlelwane be-metabolites kunye nofuzo, senze i-metabolome edibeneyo kunye nohlalutyo lwe-transcriptome. Uhlalutyo lwendlela ye-KEGG yokutyebisa ibonise ukuba ii-DEGs kunye nee-DAM zaye zatyetyiswa kwiindlela ezingama-33 ze-KEGG (Umfanekiso 5A). Phakathi kwabo, indlela "phenylpropanoid biosynthesis" yayiyeyona ityetyiswe kakhulu; indlela "iphotosynthetic carbon fixation", indlela ye "flavonoid biosynthesis", indlela ye "pentose-glucuronic acid interconversion", indlela "ye-tryptophan metabolism", kunye ne "starch-sucrose metabolism" nazo zatyetyiswa kakhulu. Imephu yokudibanisa ubushushu (Umfanekiso 5B) ubonise ukuba ii-DAM ezinxulumene ne-DEGs zahlulahlulwe zaba ziindidi ezininzi, phakathi kwazo i-flavonoids yayiyeyona nxalenye inkulu, ebonisa ukuba "i-phenylpropanoid biosynthesis" indlela idlale indima ebalulekileyo kwi-hypocotyl dwarfism.
Ababhali bavakalisa ukuba uphando lwenziwe ngokungabikho naluphi na ubudlelwane bezorhwebo okanye bezemali obunokuthi buthathwe njengento enokungqubana komdla.
Zonke iimbono ezichazwe kweli nqaku zizodwa zombhali kwaye azibonakalisi iimbono zemibutho edibeneyo, abapapashi, abahleli, okanye abahlaziyi. Naziphi na iimveliso ezivavanyiweyo kweli nqaku okanye amabango enziwe ngabavelisi bazo akaqinisekiswanga okanye avunywe ngumpapashi.
Ixesha lokuposa: Mar-24-2025