Njengolunye loxinzelelo olukhulu lwe-abiotic, uxinzelelo lobushushu obuphantsi luthintela ngokumandla ukukhula kwezityalo kwaye luchaphazela kakubi isivuno kunye nomgangatho wezityalo.I-5-Aminolevulinic acid (ALA) ngumlawuli wokukhula okhoyo ngokubanzi kwizilwanyana kunye nezityalo.Ngenxa yokusebenza kwayo okuphezulu, ukungabikho kwetyhefu kunye nokuthotywa lula, isetyenziswa ngokubanzi kwinkqubo yokunyamezelana okubandayo kwezityalo.
Nangona kunjalo, uninzi lophando lwangoku olunxulumene ne-ALA lujolise ikakhulu ekulawuleni isiphelo senethiwekhi.Indlela ethile yemolekyuli yesenzo se-ALA ekunyamezeleni ukukhula okubandayo kwezityalo okwangoku ayicacanga kwaye ifuna uphando olongezelelweyo ngoosonzululwazi.
NgoJanuwari 2024, i-Horticultural Research yapapasha iphepha lophando elinesihloko esithi "I-5-Aminolevulinic Acid Iphucula Ukunyamezela Okubandayo ngokuLawula i-SlMYB4 / SlMYB88-SlGSTU43 Imodyuli yokuHlangula kwee-Oxygen Species kwiTomato" yiqela likaHu Xiaohui kwiYunivesithi yaseNyakatho-ntshona yezolimo.
Kule sifundo, i-glutathione S-transferase gene SlGSTU43 ichongiwe kwi-tomato (i-Solanum lycopersicum L.).Iziphumo zophononongo zibonise ukuba i-ALA ikhuthaza ngamandla ukubonakaliswa kwe-SlGSTU43 phantsi koxinzelelo olubandayo.Transgenic itumato imigca overexpressing SlGSTU43 ibonise ngokuphawulekayo ukwandisa reactive iintlobo oksijini scavenging umthamo kwaye ibonise ukumelana kakhulu noxinzelelo lobushushu eliphantsi, kanti SlGSTU43 imigca eziguquguqukayo babenovakalelo kuxinzelelo lobushushu obuphantsi.
Ukongeza, iziphumo zophando zibonise ukuba i-ALA ayinyusi ukunyamezela koxinzelelo lwe-mutant kuxinzelelo oluphantsi lobushushu.Ngaloo ndlela, uphando lubonisa ukuba i-SlGSTU43 iyimfuza ebalulekileyo kwinkqubo yokuphucula ukunyamezela okubandayo kwi-tomato nge-ALA (umzobo 1).
Ukongezelela, olu pho nonongo luqinisekisiwe nge-EMSA, i-Y1H, i-LUC kunye ne-ChIP-qPCR ukufumanisa ukuba i-SlMYB4 kunye ne-SlMYB88 inokulawula ukubonakaliswa kwe-SLGSTU43 ngokuzibophelela kumgqugquzeli we-SlGSTU43.Uvavanyo olongezelelweyo lubonise ukuba i-SlMYB4 kunye ne-SlMYB88 nazo zibandakanyeka kwinkqubo ye-ALC ngokunyusa ukunyamezela kwe-tomato kuxinzelelo oluphantsi lweqondo lokushisa kunye nokulawula ngokufanelekileyo ukubonakaliswa kwe-SLGSTU43 (umzobo 2).Ezi ziphumo zibonelela ngengqiqo entsha kwindlela apho i-ALA iphakamisa ukunyamezela kuxinzelelo oluphantsi lobushushu kwitumata.
Olunye ulwazi: UZhengda Zhang et al., I-5-aminolevulinic acid iphakamisa ukunyamezela okubandayo ngokulawula imodyuli ye-SlMYB4/SlMYB88-SlGSTU43 yeentlobo ze-oksijini ezisebenzayo ezixutywayo kwi-tomato, i-Horticulture Research (2024).I-DOI: 10.1093/yure/uhae026
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Ixesha lokuposa: Jul-22-2024