Njengenye yezona zinto ziphambili ezibangela ukungondleki kakuhle, uxinzelelo oluphantsi kubushushu luthintela kakhulu ukukhula kwezityalo kwaye luchaphazela kakubi isivuno kunye nomgangatho wezityalo. I-5-Aminolevulinic acid (ALA) ngumlawuli wokukhula okhoyo kakhulu kwizilwanyana nakwizityalo. Ngenxa yokusebenza kwayo kakuhle, ukungabi natyhefu kunye nokuwohloka okulula, isetyenziswa kakhulu kwinkqubo yokunyamezela ubanda kwezityalo.
Nangona kunjalo, uninzi lophando lwangoku olunxulumene ne-ALA lugxile kakhulu ekulawuleni i-network endpoints. Indlela ethile yokusebenza kwe-ALA kwi-molecular ekunyamezeleni umkhuhlane kwasekuqaleni kwezityalo ayicaci okwangoku kwaye ifuna uphando olongezelelweyo ngososayensi.
NgoJanuwari 2024, iHorticultural Research yapapasha iphepha lophando elinesihloko esithi “5-Aminolevulinic Acid Yonyusa Ukunyamezelana Nomkhuhlane ngokulawula iSlMYB4/SlMYB88-SlGSTU43 Reactive Oxygen Species Scavenging Module in Tomato” liqela likaHu Xiaohui kwiYunivesithi yaseNorthwestern kwezolimo namahlathi.
Kolu phononongo, i-glutathione S-transferase gene SlGSTU43 ichongiwe kwitamatisi (Solanum lycopersicum L.). Iziphumo zophando zibonise ukuba i-ALA ikhuthaza kakhulu ukuvezwa kwe-SlGSTU43 phantsi koxinzelelo lokubanda. Imigca yetamatisi eguqulweyo edlulayo i-SlGSTU43 ibonise ukwanda okukhulu kwamandla okususa ioksijini kwaye ibonise ukumelana okukhulu noxinzelelo lobushushu obuphantsi, ngelixa imigca ye-SlGSTU43 mutant yayinovelwano kuxinzelelo lobushushu obuphantsi.
Ukongeza, iziphumo zophando zibonise ukuba i-ALA ayinyusi ukunyamezelana kohlobo lwe-mutant ukuya kuxinzelelo lobushushu obuphantsi. Ngoko ke, uphando lubonisa ukuba i-SlGSTU43 yijini ebalulekileyo kwinkqubo yokuphucula ukunyamezelana nokubanda kwitumato yi-ALA (Umzobo 1).
Ukongeza, olu phononongo luqinisekisile ngokufumanisa i-EMSA, i-Y1H, i-LUC kunye ne-ChIP-qPCR ukuba i-SlMYB4 kunye ne-SlMYB88 zinokulawula ukubonakaliswa kwe-SlGSTU43 ngokuzibophelela kwi-promoter ye-SlGSTU43. Uvavanyo olongezelelweyo lubonise ukuba i-SlMYB4 kunye ne-SlMYB88 nazo zibandakanyeka kwinkqubo ye-ALC ngokunyusa ukunyamezela iitumato kuxinzelelo oluphantsi lobushushu kunye nokulawula kakuhle ukubonakaliswa kwe-SlGSTU43 (Umzobo 2). Ezi ziphumo zibonelela ngokuqonda okutsha kwindlela i-ALA ephucula ngayo ukunyamezela ukunyamezela uxinezeleko oluphantsi lobushushu kwitumato.
Ulwazi oluthe kratya: Zhengda Zhang et al., i-5-aminolevulinic acid yonyusa ukunyamezelana nokubanda ngokulawula imodyuli ye-SlMYB4/SlMYB88-SlGSTU43 yokuqokelela iintlobo zeoksijini ezisabelayo kwiitamati, Uphando lwezoLimo (2024). I-DOI: 10.1093/iyure/uhae026
Ukuba udibana nengxaki yokubhala, ukungachaneki, okanye ungathanda ukufaka isicelo sokuhlela umxholo kweli phepha, nceda usebenzise le fomu. Kwimibuzo ngokubanzi, nceda sebenzisa ifomu yethu yoqhagamshelwano. Ukuze ufumane ingxelo ngokubanzi, nceda sebenzisa icandelo lezimvo zoluntu elingezantsi (landela izikhokelo).
Ingxelo yakho ibaluleke kakhulu kuthi. Nangona kunjalo, ngenxa yobuninzi bemiyalezo, asinakuqinisekisa impendulo eyenzelwe wena.
Idilesi yakho ye-imeyile isetyenziselwa kuphela ukuxelela abamkeli ukuba ngubani othumele i-imeyile. Idilesi yakho okanye idilesi yommkeli ayizukusetyenziselwa nayiphi na enye injongo. Ulwazi olufakileyo luya kuvela kwi-imeyile yakho kwaye aluyi kugcinwa yiPhys.org nangayiphi na indlela.
Fumana uhlaziyo lweveki nganye kunye/okanye lwemihla ngemihla kwibhokisi yakho yemiyalezo engenayo. Ungazikhupha nanini na kwaye asisayi kwabelana ngeenkcukacha zakho nabantu besithathu.
Senza umxholo wethu ufikeleleke kuye wonke umntu. Cinga ngokuxhasa umsebenzi weSayensi X ngeakhawunti yeprimiyamu.
Ixesha leposi: Julayi-22-2024