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I-Triacontanol ilawula ukunyamezela kwamakhukhamba kuxinzelelo lwetyuwa ngokuguqula isimo se-physiological and biochemical of cells plant.

Phantse i-7.0% yomhlaba wonke wehlabathi uchatshazelwa yityuwa1, nto leyo ethetha ukuba ngaphezu kwe-900 yezigidi zeehektare zomhlaba ehlabathini zichatshazelwa yityuwa kunye ne-sodic salinity2, ebalelwa kuma-20% omhlaba olinywayo kunye ne-10% yomhlaba onkcenkceshelwayo. ithatha isiqingatha sommandla kwaye inetyuwa ephezulu3. Umhlaba onetyuwa yingxaki enkulu ejongene nezolimo zasePakistan4,5. Koku, malunga ne-6.3 yezigidi zeehektare okanye i-14% yomhlaba onkcenkceshelwayo okwangoku ichatshazelwa yityuwa6.
Uxinzelelo lweAbiotic lunokutshintshaihomoni yokukhula kwezityaloimpendulo, ekhokelela ekuncipheni kokukhula kwesityalo kunye nesivuno sokugqibela7. Xa izityalo zibonakaliswe kuxinzelelo lwetyuwa, ibhalansi phakathi kweentlobo ze-oxygen esebenzayo (ROS) kunye nefuthe lokucima i-antioxidant enzymes liphazamisekile, okubangela ukuba izityalo zihlupheke kwi-oxidative stress8. Izityalo ezinezinga eliphezulu le-antioxidant enzymes (zombini ezisebenzayo kunye ne-inducible) zinokumelana okunempilo kumonakalo oxidative, njenge-superoxide dismutase (SOD), i-guaiacol peroxidase (POD), i-peroxidase-catalase (CAT), i-ascorbate peroxidase (APOX), kunye ne-glutathione reductase. (GR) inokunyusa ukunyamezela ityuwa yezityalo phantsi koxinzelelo lwetyuwa9. Ukongezelela, i-phytohormones iye yaxelwa ukuba idlala indima yokulawula ekukhuleni kwezityalo kunye nophuhliso, ukufa kweeseli ezicwangcisiweyo, kunye nokuphila phantsi kokutshintsha kweemeko zendalo10. I-Triacontanol i-alcohol ehluthisiweyo esisiseko esiyinxalenye ye-wax ye-epidermal yezityalo kwaye ineempawu zokukhuthaza ukukhula kwezityalo11,12 kunye neempawu zokukhuthaza ukukhula kwiindawo eziphantsi13. Ukusetyenziswa kwe-Foliar kunokuphucula kakhulu imeko ye-photosynthetic pigment, i-solute accumulation, ukukhula, kunye nokuveliswa kwe-biomass kwizityalo14,15. Ukusetyenziswa kwe-Foliar ye-triacontanol kunokuphucula ukunyamezela uxinzelelo lwezityalo16 ngokulawula umsebenzi we-antioxidant enzymes17 ezininzi, ukwandisa umxholo we-osmoprotectant we-tissue yeqabunga lezityalo11,18,19 kunye nokuphucula impendulo yokuthatha amaminerali abalulekileyo i-K + kunye ne-Ca2 +, kodwa kungekhona i-Na +. 14 Ukongezelela, i-triacontanol ivelisa ngakumbi ukunciphisa ishukela, iiprotheni ezinyibilikayo, kunye ne-amino acids phantsi kweemeko zoxinzelelo20,21,22.
Imifuno zizityebi kwii-phytochemicals kunye nezondlo kwaye ziyimfuneko kwiinkqubo ezininzi ze-metabolic kumzimba womntu23. Imveliso yemifuno isesichengeni kukwanda kwetyuwa yomhlaba, ngakumbi kwimihlaba enkcenkceshelwayo yezolimo, evelisa i-40.0% yokutya kwehlabathi24. Izityalo zemifuno ezifana netswele, cucumber, eggplant, ipepile kunye netumata zinobuntununtunu kwityuwa25, kwaye ikhukhamba yimifuno ebalulekileyo kwisondlo somntu kwihlabathi jikelele26. Uxinzelelo lwetyuwa lunefuthe elibonakalayo kwisantya sokukhula kwekhukhamba, nangona kunjalo, amanqanaba etyuwa ngaphezulu kwe-25 mM akhokelela ekunciphiseni kwesivuno ukuya kutsho kwi-13% 27,28. Iziphumo ezinobungozi zetyuwa kwicucumber zikhokelela ekukhuleni kwesityalo esinciphileyo kunye nesivuno5,29,30. Ngoko ke, injongo yolu phononongo yayikukuvavanya indima ye-triacontanol ekunciphiseni uxinzelelo lwetyuwa kwi-genotypes ye-cucumber kunye nokuvavanya amandla e-triacontanol ukukhuthaza ukukhula kwezityalo kunye nemveliso. Olu lwazi lukwabalulekile ekuphuhliseni amacebo alungele umhlaba onetyuwa. Ukongezelela, sinqume utshintsho kwi-ion homeostasis kwi-cucumber genotypes phantsi koxinzelelo lwe-NaCl.
Impembelelo ye-triacontanol kwi-inorganic osmotic regulators kumagqabi amane e-cucumber genotypes phantsi koxinzelelo oluqhelekileyo kunye netyuwa.
Xa i-cucumber genotypes yahlwayelwa phantsi kweemeko zoxinzelelo lwetyuwa, inani elipheleleyo lesiqhamo kunye nobunzima besiqhamo buncitshiswe kakhulu (umzobo 4). Oku kuncitshiswa kwacaca ngakumbi kwi-Summer Green kunye ne-20252 genotypes, ngelixa i-Marketmore kunye ne-Green Long zigcine inani eliphezulu leziqhamo kunye nobunzima emva komngeni wetyuwa. Ukusetyenziswa kwe-Foliar ye-triacontanol yanciphisa imiphumo emibi yoxinzelelo lwetyuwa kunye nokwanda kwenani leziqhamo kunye nobunzima kuzo zonke i-genotypes ezivavanyiweyo. Nangona kunjalo, i-Marketmore enyangwa nge-triacontanol ivelise elona nani liphezulu leziqhamo ezinobunzima obuphakathi obuphezulu phantsi koxinzelelo kunye neemeko ezilawulwayo xa kuthelekiswa nezityalo ezinganyangwanga. I-Summer Green kunye ne-20252 yayinomxholo ophezulu we-soluble solids kwiziqhamo ze-cucumber kwaye isebenze kakubi xa ithelekiswa ne-Marketmore kunye ne-Green Long genotypes, eyayinoxinzelelo oluphantsi lulonke lwe-soluble solids.
Impembelelo ye-triacontanol kwisivuno se-cucumber genotypes ezine phantsi kweemeko eziqhelekileyo kunye noxinzelelo lwetyuwa.
Uxinzelelo oluphezulu lwe-triacontanol lwaluyi-0.8 mg / l, eyavumela ukunciphisa imiphumo ebulalayo ye-genotypes efundwayo phantsi koxinzelelo lwetyuwa kunye neemeko ezingenaxinzelelo. Nangona kunjalo, umphumo we-triacontanol kwiGreen-Long kunye ne-Marketmore wawucace ngakumbi. Ukuqwalasela amandla okunyamezela ityuwa kwezi genotypes kunye nokusebenza kwe-triacontanol ekudambiseni iziphumo zoxinzelelo lwetyuwa, kunokwenzeka ukucebisa ukukhulisa ezi genotypes kwimihlaba enetyuwa ngokutshiza ngamahlamvu nge-triacontanol.

 

Ixesha lokuposa: Nov-27-2024