Yintoni umahluko phakathi kwe-zeatin, i-Trans-zeatin kunye ne-zeatin riboside? Zithini iindlela ezisetyenziswa ngazo?

Imisebenzi ephambili

1. Khuthaza ukwahlukana kweeseli, ikakhulu ukwahlukana kwe-cytoplasm;

2. Khuthaza ukwahlulwahlulwa kwamahlumela. Kwinkcubeko yezicubu, isebenzisana ne-auxin ukulawula ukwahlulwahlulwa kunye nokwakheka kweengcambu kunye namahlumela;

3. Khuthaza uphuhliso lwamahlumela asecaleni, ukuphelisa ukubusa kwe-apical, ngaloo ndlela kukhokelele ekwakhiweni kwenani elikhulu lamahlumela angena kwinkcubeko yezicubu;

4. Ukulibazisa ukuguga kwamagqabi, ukucothisa izinga lokuwohloka kwe-chlorophyll kunye neeproteni;

5. Yaphula imbewu ukuze ingakhuli, tshintsha isibane ukuze ihlangabezane neemfuno zokukhanya zembewu efana necuba;

6. Yenza i-parthenocarpy kwiziqhamo ezithile;

7. Khuthaza ukwakheka kweziqalo zamahlumela: kwiziphelo zamagqabi ezisikiweyo nakwezinye iimosses, inokukhuthaza ukwakheka kweziqalo zamahlumela;

8. Khuthaza ukwakheka kweengcambu zeetapile.

Iqulethe kuphela isakhiwo se-trans kwaye inesiphumo esifanayo nesoi-zeatin, kodwa ngomsebenzi onamandla ngakumbi.

Isiphumo sayo sifana kakhulu neso se-anti-zeatin. Ayinamsebenzi we-zeatin okhankanywe apha ngasentla kuphela, kodwa ikwanayo nesiphumo sokuvuselela ukubonakaliswa kwezakhi zofuzo kunye nomsebenzi we-metabolic.

Indlela Yokusebenzisa

1. Khuthaza ukuhluma kwe-callus (kufuneka isetyenziswe kunye ne-auxin), uxinzelelo lwe-1mg/L.

2. Khuthaza ukumiselwa kweziqhamo, i-1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, faka iziqhamo emva kweentsuku ezili-10, ezingama-25, kunye nama-40 emva kokudubula.

3. Kwimifuno enamagqabi, fafaza nge-201 mg/L ukuze ubambezele ukutyheli kwamagqabi.

Ukongeza, ukunyanga ezinye iimbewu zezityalo kunokukhuthaza ukuhluma; ukunyanga kwisigaba sokuhluma kwezithole kunokukhuthaza ukukhula.

1. Khuthaza ukuhluma kwezicubu ze-callus (kufuneka zisetyenziswe kunye ne-auxin), kuxinzelelo lwe-1 ppm;

2. Khuthaza ukumiselwa kweziqhamo, i-100 ppm ye-cytokinin + 500 ppm ye-GA3 + 20 ppm ye-NAA, fafaza iziqhamo kwiintsuku ezili-10, ezingama-25, kunye nama-40 emva kokudubula;

3. Libazisa ukutyheli kwamagqabi emifuno, fafaza nge-20 ppm;

1. Kwinkcubeko yezicubu zezityalo, uxinaniso oluqhelekileyo lwe-anti-cytokinin nucleoside yi-1 mg/mL okanye ngaphezulu.

2. Kwimithetho yokukhula kwezityalo, uxinaniso lwe-anti-cytokinin nucleoside ludla ngokuba yi-1 ppm ukuya kwi-100 ppm, kwaye uxinaniso oluthile luxhomekeke kwisicelo esithile kunye neentlobo zezityalo. Umzekelo, xa kukhuthazwa ukuhluma kwezicubu ze-callus, uxinaniso lwe-anti-cytokinin nucleoside yi-1 ppm, kwaye kufuneka isetyenziswe kunye ne-auxin.

3. Nyibilikisa umgubo we-anti-cytokinin nucleoside kakuhle nge-2-5 mL ye-1 M NaOH (okanye i-1 M acetic acid okanye i-1 M KOH), uze wongeze amanzi axutyiweyo kabini okanye amanzi acocekileyo kakhulu ukulungiselela isisombululo sokugcina se-1 mg/mL okanye uxinzelelo oluphezulu. Xuba ngelixa ufaka amanzi ukuqinisekisa ukuxubana kakuhle. Isisombululo sokugcina kufuneka sixutywe kwaye sigcinwe siqandisiwe ukuze kuthintelwe ukuqandisa okuphindaphindiweyo. Nyibilikisa isisombululo sokugcina kunye nendawo yokukhulisa ukuya kuxinzelelo olufunekayo, kwaye ulungiselele isisombululo esisebenzayo kwangoko kwaye usisebenzise ngoko nangoko.

Ukuqukumbela, i-zeatin, i-abscisic acid kunye ne-abscisic acid nucleotide nganye ineempawu zayo ngokwesakhiwo, umsebenzi kunye nokusetyenziswa kwayo. Ukuqukumbela, i-zeatin, i-abscisic acid kunye ne-abscisic acid nucleotide nganye ineempawu zayo ngokwesakhiwo, umsebenzi kunye nokusetyenziswa kwayo. Nangona kunjalo, zonke zisebenza njengabalawuli bokukhula kwezityalo kwaye zidlala indima ebalulekileyo ekukhuthazeni ukukhula nophuhliso lwezityalo.