Yintoni umahluko phakathi kwe-zeatin, i-Trans-zeatin kunye ne-zeatin riboside? Ziziphi izicelo zabo?

Imisebenzi ephambili

1. Ukukhuthaza ukwahlukana kweeseli, ngokukodwa ukwahlukana kwe-cytoplasm;

2. Khuthaza ukwahluka kwamahlumelo. Kwinkcubeko ye-tissue, isebenzisana ne-auxin ukulawula ukuhlukana kunye nokwakhiwa kweengcambu kunye neengcambu;

3. Ukukhuthaza ukuphuhliswa kwee-lateral buds, ukuphelisa ukubusa kwe-apical, kwaye ngaloo ndlela kukhokelela ekubunjweni kwenani elikhulu le-adventitious buds kwinkcubeko ye-tissue;

4. Ukulibaziseka kwamagqabi, ukuthothisa izinga lokuthotywa kweklorofili kunye neeproteni;

5. Yaphula i-dormancy yembewu, buyisela isibane ukuhlangabezana neemfuno zokukhanya zembewu efana necuba;

6. Ukukhuthaza i-parthenocarpy kwiziqhamo ezithile;

7. Ukukhuthaza ukubunjwa kwama-initials e-bud: kwiindawo ezinqamlekileyo zamagqabi kunye nakweminye i-mosses, inokukhuthaza ukubunjwa kwee-initials ze-bud;

8. Ukuvuselela ukubunjwa kwee-tubers zetapile.

Iqulethe kuphela i-trans structure kwaye inefuthe elifanayo njengezeatin, kodwa ngomsebenzi owomeleleyo.

Impembelelo yayo ifana kakhulu ne-anti-zeatin. Ayinayo nje le misebenzi ikhankanywe apha ngasentla ye-zeatin, kodwa inefuthe lokuvula i-gene expression kunye nomsebenzi we-metabolic.

Indlela yokusebenzisa

1. Ukukhuthaza ukuhluma kwe-callus (kufuneka isetyenziswe ngokudibanisa ne-auxin), i-concentration 1mg / L.

2. Ukukhuthaza ukusekwa kweziqhamo, 1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, fafaza kwiziqhamo ze-10, 25, kunye neentsuku ezingama-40 emva kokutyatyamba.

3. Kwimifuno enamagqabi, fafaza nge-201 mg/L ukulibazisa ukuvela kwegqabi.

Ukongeza, ukunyanga ezinye iimbewu zesityalo kunokukhuthaza ukuntshula; ukunyanga kwinqanaba lezithole kunokukhuthaza ukukhula.

1. Ukukhuthaza ukuhluma kwezicubu ze-callus (kufuneka zisetyenziswe ngokudibanisa ne-auxin), kwi-concentration ye-1 ppm;

2. Ukukhuthaza ukusekwa kweziqhamo, i-100 ppm ye-cytokinin + 500 ppm ye-GA3 + 20 ppm ye-NAA, fafaza iziqhamo 10, 25, kunye neentsuku ezingama-40 emva kokudubula;

3. Libazise ukutyheli kwamagqabi emifuno, fafaza i-20 ppm;

1. Kwinkcubeko yezicubu zezityalo, i-concentration ye-anti-cytokinin nucleoside yi-1 mg / mL okanye ngaphezulu.

2. Kulawulo lokukhula kwezityalo, ukuxinwa kwe-anti-cytokinin nucleoside ngokuqhelekileyo yi-1 ppm ukuya kwi-100 ppm, kwaye ukugxininiswa okuthe ngqo kuxhomekeke kwisicelo esithile kunye neentlobo zezityalo. Ngokomzekelo, xa ukhuthaza ukuhluma kwezicubu ze-callus, ukuxinwa kwe-anti-cytokinin nucleoside yi-1 ppm, kwaye kufuneka isetyenziswe ngokudibanisa ne-auxin.

3. Dissolve i-anti-cytokinin nucleoside powder ngokucokisekileyo kunye ne-2-5 mL ye-1 M NaOH (okanye i-1 M acetic acid okanye i-1 M KOH), uze ungeze amanzi adibeneyo aphindwe kabini okanye i-ultrapure isisombululo sokugcina isisombululo se-1 mg / mL okanye i-concentration ephezulu. Gxuma ngelixa ufaka amanzi ukuqinisekisa ukuxuba ngokucokisekileyo. Isisombululo sogcino kufuneka sincitshiswe kwaye sifakwe emkhenkceni ukuphepha ukuphinda kunyibilike umkhenkce. Nciphisa isisombululo sokugcina kunye nenkcubeko yenkcubeko ukuya kugxininiso olufunekayo, kwaye ulungiselele isisombululo sokusebenza kwindawo kwaye uyisebenzise ngokukhawuleza.

Ekugqibeleni, i-zeatin, i-abscisic acid kunye ne-abscisic acid nucleotide nganye ineempawu zayo ngokubhekiselele kwisakhiwo, umsebenzi kunye nezicelo ezisebenzayo.Ekugqibeleni, i-zeatin, i-abscisic acid kunye ne-abscisic acid nucleotide nganye ineempawu zayo ngokubhekiselele kwisakhiwo, umsebenzi kunye nokusetyenziswa okusebenzayo. Nangona kunjalo, zonke zisebenza njengabalawuli bokukhula kwezityalo kwaye zidlala indima ebalulekileyo ekukhuthazeni ukukhula nophuhliso lwezityalo.