Ngemveliso yonyaka engaphezulu kwe-700,000 yeetoni, i-glyphosate yeyona nto isetyenziswa kakhulu kwaye inkulu emhlabeni. Ukuchasana nokhula kunye neengozi ezinokubakho kwimeko yendalo kunye nempilo yabantu ebangelwa ukusetyenziswa kakubi kwe-glyphosate kuye kwatsala ingqalelo enkulu.
Ngomhla wama-29 kuMeyi, iqela likaNjingalwazi uGuo Ruiting elivela kwiLabhoratri yeSizwe ye-Biocatalysis kunye neNjineli ye-Enzyme, ngokudibeneyo esekwe yiSikolo soBomi beeSayensi zeYunivesithi yaseHubei kunye namasebe ephondo kunye nabaphathiswa, bapapashe iphepha lophando lwamva nje kwi-Journal of Hazardous Materials, ukuhlalutya uhlalutyo lokuqala lwengca ye-barnyard. (Ukhula olubi lwe-paddy) -ephuma kwi-aldo-keto reductase AKR4C16 kunye ne-AKR4C17 i-catalyze the reaction mechanism of glyphosate degradation, kwaye iphucule kakhulu ukuthotywa kokusebenza kwe-glyphosate nge-AKR4C17 ngokuguqulwa kwemolekyuli.
Ukukhula ukumelana ne-glyphosate.
Ukusukela oko yathi yaziswa ngeminyaka yoo-1970, iglyphosate ibisaziwa kwihlabathi liphela, kwaye ngokuthe ngcembe iye yaba yeyona inexabiso eliphantsi, esetyenziswa kakhulu kwaye inemveliso ebanzi yokutshabalalisa ukhula. Ibangela ukuphazamiseka kwe-metabolic kwizityalo, kubandakanywa nokhula, ngokuthintela ngokukodwa i-5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), i-enzyme ephambili ebandakanyekayo ekukhuleni kwezityalo kunye ne-metabolism. kunye nokufa.
Ke ngoko, ukuzalanisa izityalo ze-glyphosate ezinganyangekiyo kunye nokusebenzisa i-glyphosate entsimini yindlela ebalulekileyo yokulawula ukhula kwezolimo zanamhlanje.
Nangona kunjalo, ngokusetyenziswa ngokubanzi kunye nokusetyenziswa kakubi kwe-glyphosate, ukhula oluninzi luye lwavela ngokuthe ngcembe kwaye lwaphuhlisa ukunyamezela okuphezulu kwe-glyphosate.
Ukongezelela, izityalo eziguquguqukayo ze-glyphosate azikwazi ukubola i-glyphosate, okubangela ukuqokelela kunye nokudluliselwa kwe-glyphosate kwizityalo, ezinokusasazeka ngokulula kwikhonkco lokutya kunye nokubeka impilo yabantu engozini.
Ke ngoko, kungxamisekile ukufumanisa iigenes ezinokuthi zithobe i-glyphosate, ukuze kulinywe izityalo ezichasene ne-glyphosate ezichasene ne-glyphosate kunye neentsalela ze-glyphosate eziphantsi.
Ukusombulula ubume bekristale kunye ne-catalytic reaction mechanism ye-glyphosate-degrading enzymes ephuma kwisityalo
Ngo-2019, amaqela ophando aseTshayina nase-Australia achonge i-aldo-keto reductase ezimbini ze-glyphosate-degrading, i-AKR4C16 kunye ne-AKR4C17, okokuqala ngqa kwi-glyphosate-resistant barnyard grass. Bangasebenzisa i-NADP+ njenge-cofactor ukuthoba i-glyphosate kwi-nontoxic aminomethylphosphonic acid kunye ne-glyoxylic acid.
I-AKR4C16 kunye ne-AKR4C17 yi-enzyme yokuqala echazwe kwi-glyphosate-degrading enzymes eveliswa yindalo yendalo yezityalo. Ukwenzela ukuphonononga ngakumbi indlela yemolekyuli yokuthotywa kwabo kweglyphosate, iqela likaGuo Ruiting lasebenzisa iX-ray crystallography ukuhlalutya ubudlelwane phakathi kwezi enzymes zimbini kunye necofactor ephezulu. Ulwakhiwo oluntsonkothileyo lwesisombululo luveze indlela yokubophelela kwe-ternary complex ye-glyphosate, i-NADP+ kunye ne-AKR4C17, kwaye indulule indlela yokusabela ye-catalytic ye-AKR4C16 kunye ne-AKR4C17-mediated glyphosate degradation.
Ulwakhiwo lwe-AKR4C17/NADP+/glyphosate entsonkothileyo kunye ne-reaction mechanism ye-glyphosate degradation.
Ukuguqulwa kweemolekyuli kuphucula ukusebenza kakuhle kokuthotywa kwe-glyphosate.
Emva kokufumana imodeli yolwakhiwo oluhle lwe-AKR4C17/NADP+/glyphosate, iqela likaNjingalwazi uGuo Ruiting liphinde lafumana iprotein eguqukileyo i-AKR4C17F291D ngokunyuka kwe-70% ekuthotyweni kokuthotywa kweglyphosate ngohlalutyo lwesakhiwo se-enzyme kunye noyilo olunengqiqo.
Uhlalutyo lomsebenzi we-glyphosate-degrading of AKR4C17 mutants.
"Umsebenzi wethu utyhila indlela yeemolekyuli ze-AKR4C16 kunye ne-AKR4C17 ebangela ukuthotywa kwe-glyphosate, ebeka isiseko esibalulekileyo sohlengahlengiso olongezelelweyo lwe-AKR4C16 kunye ne-AKR4C17 ukuphucula ukusebenza kwabo kokuthotywa kwe-glyphosate." Umbhali wephepha, uNjingalwazi uDai Longhai weYunivesithi yaseHubei uthe bakhe iprotein eguqukileyo i-AKR4C17F291D ngophuculo oluphuculweyo lokuthotywa kweglyphosate, ebonelela ngesixhobo esibalulekileyo sokulima izityalo ze-glyphosate ezixhathisayo kunye neentsalela zeglyphosate eziphantsi kunye nokusebenzisa ubunjineli be-glyphosate ye-microbial.
Kuxelwe ukuba iqela likaGuo Ruiting kudala lizibandakanyile kuphando kuhlalutyo lwesakhiwo kunye nengxoxo yendlela yee-enzymes ze-biodegradation, i-terpenoid synthases, kunye neeproteni ekujoliswe kuzo kumachiza ezinto eziyityhefu neziyingozi kwindalo esingqongileyo. U-Li Hao, umphandi onxulumene noYang Yu kunye nomhlohli u-Hu Yumei kwiqela ngababhali bokuqala bephepha, kwaye uGuo Ruiting kunye noDai Longhai ngababhali abahambelanayo.
Ixesha lokuposa: Jun-02-2022