Ngemveliso yonyaka engaphezulu kwe-700,000 yeetoni, i-glyphosate yeyona nto isetyenziswa kakhulu kwaye inkulu emhlabeni.Ukuchasana nokhula kunye nezisongelo ezinokuthi zibekho kwimeko yendalo kunye nempilo yabantu ebangelwa ukusetyenziswa kakubi kwe-glyphosate kuye kwatsala ingqalelo enkulu.
Ngomhla wama-29 kaMeyi, iqela likaNjingalwazi uGuo Ruiting elivela kwiLabhoratri yeSizwe yeBiocatalysis kunye nobuNjineli beNzyme, ngokudibeneyo esekwe yiSikolo soBomi beSayensi yeYunivesithi yaseHubei kunye namasebe ephondo kunye nabaphathiswa, yapapasha iphepha lophando lwamva nje kwiJournal of Hazardous Materials, ukuhlalutya. uhlalutyo lokuqala lwengca yasebarnyard.(Ukhula olubi lwe-paddy) -ephuma kwi-aldo-keto reductase AKR4C16 kunye ne-AKR4C17 i-catalyze the reaction mechanism of glyphosate degradation, kwaye iphucule kakhulu ukuthotywa kokusebenza kwe-glyphosate nge-AKR4C17 ngokuguqulwa kwemolekyuli.
Ukukhula ukumelana ne-glyphosate.
Ukusukela oko yathi yaziswa ngeminyaka yoo-1970, iglyphosate ibisaziwa kwihlabathi liphela, kwaye ngokuthe ngcembe iye yaba yeyona inexabiso eliphantsi, esetyenziswa kakhulu kwaye inemveliso ebanzi yokutshabalalisa ukhula.Ibangela ukuphazamiseka kwe-metabolic kwizityalo, kubandakanywa nokhula, ngokuthintela ngokukodwa i-5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), i-enzyme ephambili ebandakanyekayo ekukhuleni kwezityalo kunye ne-metabolism.kunye nokufa.
Ke ngoko, ukuzalanisa izityalo ze-glyphosate ezinganyangekiyo kunye nokusebenzisa i-glyphosate entsimini yindlela ebalulekileyo yokulawula ukhula kwezolimo zanamhlanje.
Nangona kunjalo, ngokusetyenziswa ngokubanzi kunye nokusetyenziswa kakubi kwe-glyphosate, ukhula oluninzi luye lwavela ngokuthe ngcembe kwaye lwaphuhlisa ukunyamezela okuphezulu kwe-glyphosate.
Ukongezelela, izityalo eziguquguqukayo ze-glyphosate azikwazi ukubola i-glyphosate, okubangela ukuqokelela kunye nokudluliselwa kwe-glyphosate kwizityalo, ezinokusasazeka ngokulula kwikhonkco lokutya kunye nokubeka impilo yabantu engozini.
Ke ngoko, kungxamisekile ukufumanisa iigenes ezinokuthi zithobe i-glyphosate, ukuze kulinywe izityalo ezichasene ne-glyphosate ezichasene ne-glyphosate kunye neentsalela ze-glyphosate eziphantsi.
Ukusombulula ubume bekristale kunye ne-catalytic reaction mechanism ye-glyphosate-degrading enzymes ephuma kwisityalo
Ngo-2019, amaqela ophando aseTshayina nase-Australia achonge i-aldo-keto reductase ezimbini ze-glyphosate-degrading, i-AKR4C16 kunye ne-AKR4C17, okokuqala ngqa kwi-glyphosate-resistant barnyard grass.Bangasebenzisa i-NADP+ njenge-cofactor ukuthoba i-glyphosate kwi-nontoxic aminomethylphosphonic acid kunye ne-glyoxylic acid.
I-AKR4C16 kunye ne-AKR4C17 yi-enzyme yokuqala echazwe kwi-glyphosate-degrading enzymes eveliswa yindalo yendalo yezityalo.Ukwenzela ukuphonononga ngakumbi indlela yemolekyuli yokuthotywa kwabo kweglyphosate, iqela likaGuo Ruiting lasebenzisa iX-ray crystallography ukuhlalutya ubudlelwane phakathi kwezi enzymes zimbini kunye necofactor ephezulu.Ulwakhiwo oluntsonkothileyo lwesisombululo luveze indlela yokubophelela kwe-ternary complex ye-glyphosate, i-NADP+ kunye ne-AKR4C17, kwaye indulule indlela yokusabela ye-catalytic ye-AKR4C16 kunye ne-AKR4C17-mediated glyphosate degradation.
Ulwakhiwo lwe-AKR4C17/NADP+/glyphosate entsonkothileyo kunye ne-reaction mechanism ye-glyphosate degradation.
Ukuguqulwa kweemolekyuli kuphucula ukusebenza kakuhle kokuthotywa kwe-glyphosate.
Emva kokufumana imodeli yolwakhiwo oluhle lwe-AKR4C17/NADP+/glyphosate, iqela likaNjingalwazi uGuo Ruiting liphinde lafumana iprotein eguqukileyo i-AKR4C17F291D ngokunyuka kwe-70% ekuthotyweni kokuthotywa kweglyphosate ngohlalutyo lwesakhiwo se-enzyme kunye noyilo olunengqiqo.
Uhlalutyo lomsebenzi we-glyphosate-degrading of AKR4C17 mutants.
"Umsebenzi wethu utyhila indlela yeemolekyuli ze-AKR4C16 kunye ne-AKR4C17 ebangela ukuthotywa kwe-glyphosate, ebeka isiseko esibalulekileyo sohlengahlengiso olongezelelweyo lwe-AKR4C16 kunye ne-AKR4C17 ukuphucula ukusebenza kwabo kokuthotywa kwe-glyphosate."Umbhali wephepha, uNjingalwazi uDai Longhai weYunivesithi yaseHubei uthe bakhe iprotein eguqukayo i-AKR4C17F291D ngophuculo oluphuculweyo lokuthotywa kweglyphosate, ebonelela ngesixhobo esibalulekileyo sokulima izityalo eziphezulu ze-glyphosate ezinganyangekiyo kunye neentsalela zeglyphosate eziphantsi kunye nokusebenzisa ibhaktheriya yobunjineli be-microbial. ukuthoba i-glyphosate kwimekobume.
Kuxelwe ukuba iqela likaGuo Ruiting kudala lizibandakanyile kuphando kuhlalutyo lwesakhiwo kunye nengxoxo yendlela yee-enzymes ze-biodegradation, i-terpenoid synthases, kunye neeproteni ekujoliswe kuzo kumachiza ezinto eziyityhefu neziyingozi kwindalo esingqongileyo.U-Li Hao, umphandi onxulumene noYang Yu kunye nomhlohli u-Hu Yumei kwiqela ngababhali bokuqala bephepha, kwaye uGuo Ruiting kunye noDai Longhai ngababhali abahambelanayo.
Ixesha lokuposa: Jun-02-2022